ENHANCED BIOFUELS AND TECHNOLOGIES INDIA (P) LTD
&
THE STATE TRADING CORPORATION OF INDIA LIMITED PROJECT PROFILE OVERVIEW We, Enhanced Biofuels & Technologies India (P) Ltd., feel proud to introduce ourselves as one of the pioneers in the field of Biofuel production using Non edible Sources of feed stock like Jatropha and Algae. The fine art of Natural Science combined with the Innovative Technology has brought a great success in the field of Research and Development. Our breakthrough in the field of Agronomy, processing technology, renewable energy, biotechnology, microbiology and Phycology. The company has undertaken research and development into each of these various fields, for the purpose of applying scientific methodology to the design, development, processing and distribution of alternative biofuels, the generation/distribution of bio-energy and the processing of bi-products. Biodiesel production has marked our presence in the Biofuel Market ever since 2002.We have invested $ 12 million for the research on Biofuel production. Our group of 36 Scientists and the Science Advisory Board has made everlasting achievements in the field of R & D.
The State Trading Corporation of India Ltd (STC), a premier international trading company under the Ministry of Commerce & Industry, Department of Commerce, and Government of India in association with M/s Enhanced Biofuels & Technologies (India) Pvt. Limited (EBTI) is offering Biofuels project using Non edible feed stock such as Jatropha in Algae in all parts of the world.
INTRODUCTION The State Trading Corporation of India Ltd (STC) is a premier international trading company under the Ministry of Commerce & Industry, Department of Commerce, Government of India. STC has recently celebrated its Golden Jubilee and is now entering its 52nd year of service to the nation. STC has an equity base of Indian Rupees 300 million (USD 7.5 Million), of which, 91% is held by the Government of India. In the recent years, there has been a continuous spurt in the business activities of the Corporation and STC’s turnover reached an all time high of over Indian Rupees 14.3 billion (USD 3.5 billion) in the year 2006-2007. The net worth of the Corporation is over Indian Rupees 4 Billion. STRATEGIC ALLIANCE STC has entered into a strategic alliance with Enhanced Biofuels and Technologies Limited, Coimbatore, India (EBTI) for exploring markets around the globe for supply/ cultivation of Jatropha curcas Linn planting materials, bio-fertilizers, bio-pesticides and related processing technology and equipments for production of bio-fuels. STC will support EBTI with its vast international network and alliances in setting up various initiatives including joint ventures in identified countries in Asia and around the globe. OUR ASSOCIATES – ENHANCED BIOFUELS AND TECHNOLOGIES LIMITED (EBTI) Enhanced Biofuels and Technologies Limited (EBTI) have established a presence in the field of processing technology, renewable energy, biotechnology, microbiology and Phycology. The company has undertaken research and development into each of these various fields, for the purpose of applying scientific methodology to the design, development, processing and distribution of alternative biofuels, the generation/distribution of bio-energy and the processing of bi-products. HISTORY EBTI's focus has been geared towards funding research and development for alternative energy resources, inclusive of the execution pilot projects.

COMPETENCIES EBTI has evolved a highly integrated complimentary operational profile that embraces a team of scientific and technical skills drawn from some of the world's foremost academic, industrial, policy and financial sectors. EBTI’s core physical assets centre on the scientific work that has been performed at their laboratory in
Coimbatore, India, and the virtual assets of the company span all four continents; EBTI possesses a modern day global footprint.

STRATEGY EBTI's is continuing to improve upon microbiology, biotechnology and processing techniques associated with the cultivation of Jatropha curcas Linn as a viable feedstock's for the production of bio energy. To date, the company has established cultivation benchmarks that enhance feedstock quality, productivity and yields that are accredited worldwide. PRODUCTS JATROPHA CURCAS LINN Jatropha in India & Part of EBTI in development of Jatropha Jatropha curcas is known as non edible oil seed and good feed stock for Bio Diesel world. Still it is not handled as an alternate crop (or) commercial crop in farming community. All government institutions and non governmental organizations are involved in the development program of Jatropha. Jatropha is used as fencing crop for all Agriculture crops. Few years ago, it was considered as a cultivable crop by farmers. Because of lower productivity and poor price in market, it has been eradicated. The fossil petroleum crude oil price and decreasing of crude oil availability has led to increase in the popularity of Jatropha.
Problems of Jatropha
1. Proper breeding work was not carried out
2. Low physical and genetic purity planting material was handled
3. Cultivated as rain fed crop
4. Planted in improper spacing
5. Plant nutrition was not standardized
6. Plant protection methods was not standardized & practiced
7. No training and pruning
8. Lot of Variation by cross pollination and segregations EBTI mandates
1. Multiplication and supply of high yielding clones of Jatropha
2. Isolation and standardization of protocol for tissue culture
3. Multiplication of growing media, and specific strains for Jatropha
4. Standardization of packages of practices based high yield and good quality of oil easy to convert in to Bio diesel with minimal modifications
5. Identification of high value intercrops in Jatropha according to the available facilities
6. Detoxification and standardization of protocol for photochemical isolation from cakes
7. Isolation of Bio pesticide from leaves and cakes
8. Honey collection by honey bee and identification medicinal properties from Jatropha 9. Identification of medicines from the leaves for piles and some external blood wounds EBTI has been following an intensive research program on the cultivation of Jatropha and has been successful in creating crops of high yielding varieties with high oil content and resistant to harmful pests by a careful germplasm selection focusing on closed internodes, sex ratio, fruit setting percentage, dry seed weight, and germination and seed viability. EBTI has achieved the world record break of high yielding germplasm (60 fruits / cyme)
• Identification Mite resistant line
• Identification and standardization of IPM for Mites
• Achieved 120 branches in seedling progeny in 180th day
• Getting first harvest in 50 days old plants
• Well developed kernels with all seeds
• Standardization the protocol for 95% rooting in vegetative propagation
• Standardization the Agronomical technology (Pruning, Spacing,)
• Excellent rooting and growth promotion by EBTI
• Discovered Plant growth promoting micro organism and Probiotic microbial consortium to remediate the heavy metals infected soil
• Isolation of associated micro organism for plant protection and nutrient dilution and fixation
• Isolation of sulphur solubilizing bacteria to enhance the oil recovery & Quality of the oil
• Isolation Bio digester fro Bio gas production and Coir pith composting
• Identified micro organisms for detoxification of Jatropha de oiled cake
• Proposed for sex pheromone trap for fruit sucking bug which can expected important pest in Jatropha farms Selection of variety • EBTI has explored 270 high yielding clones out of 15 million wild plants.
• Their selections yielded to a maximum of 1.8 kg seeds in 9 months and on an average of 0.9kg per plant.
• EBTI has recorded 18 bunches and average of 27 fruits per bunch with a seed weight of 0.8 g per seed (as against highest recorded globally 0.6g certified by Dr George Francis, Hogeingem University Germany).
• EBTI has identified an accession having potentiality of producing 60 and 85 fruits/bunch.
• EBTI has developed commercially high yielding clones of Jatropha curcas producing 5kg/tree with oil yield of 42% Clones producing 12kg/tree is under multi-location trial with a target of 7.9 mt of oil/hectare /yr.
• EBTI has developed pest resistant Jatropha cultivar.
• EBTI has achieved 90% seed germination and 95% root initiation in CPM (Clonal propagation Method). Identified location and species specific bio- fertilizers & bio- control agents for enhanced Jatropha curcas cultivation as Bioprime, Bioprotect and Bioprevent.
• EBTI has optimized Integrated Nutrient management, Integrated Pest Management and water requirements for Jatropha curcas.
• EBTI has standardized protocol for tissue culture Jatropha and mass multiplication of clonal propagating material.
• A revenue model with commercially proven technologies for Jatropha to generate profit as follows.
o $ 37.6 million for 5000 hectare plantation using Jatropha cake as a manure
o $ 43.4 million for 5000 hectare plantation using Jatropha cake as a Animal feed • EBTI has developed conception of Jatropha based multi-tiered intercropping system using high value medicinal and aromatic plants such as spices (Ginger & Pepper) and Vanilla. Standardization of packages of practices based on high yield and good quality of oil easy to convert into Bio diesel with minimal modifications
a. Integrated water management
b. Integrated plant protection technology
c. Integrated nutrient management
d. Integrated weed management
e. Enriched manure production by weeds
f. Phytoremediation with Jatropha
g. Using hormones to increase the female flower production
h. Coating with bio-polymer and bio-fertilizer to increase the Viability of seed
i. Training pruning methods to produce more branches
j. Precision seed production technology from clonal orchards By a well researched process, EBTI has been able to demonstrate the right farming techniques to be adopted for successful and commercially viable cultivation of Jatropha. EBTI has a technological alliance with major international partners for the oil extraction and refining and in due course of time, will be setting up joint ventures with such partnerships both in the space of commercial plantation and oil extraction and refining. As a result of research into the plant's genetic characteristics, fertilization, pesticide and physical management requirements, EBTI possesses a holistic agronomy data base for the complete cultivation of Jatropha curcas as an economically viable energy crop/feedstock for the production of bio fuels. EBTI has established significant technology advances for the processing of Jatropha curcas bio mass into high value commodities. These include: bio lubricants, pharmaceutical supplies, bio ethanol, bio energy and insect repellents, adding value and feasibility for the cultivation of Jatropha curcas as a primary crop for undeveloped land throughout Southern Asia, Africa and the Middle East.

EBTI has recognised the socio-economic value cultivation of Jatropha curcas has for the highly decentralised rural communities of developing economic regions throughout the Middle East, Asian, African, Latin American and Pacific islands. In support of commercial pro-poverty reduction policy, EBTI's executive has designed and engineered an agricultural extension system for Jatropha curcas specifically to address international policy initiatives that seek to include sub tropical agricultural activity as a factor of national fuel security.
ALGAE SOURCE OF BIOFUELS:
POND SCUM – A PROMISING SOURCE:
Microalgae appear to be the only promising source of renewable biodiesel that is capable of meeting the global demand for transport fuels. Like plants, microalgae use sunlight to produce oils but they do so more efficiently than crop plants. Oil productivity of many microalgae greatly exceeds the oil productivity of the best
producing oil crops
EBT’S FOCUS ON MICROALGAE DERIVED BIOFUELS:
Currently, EBT’s focus projects on the Biofuels derived from tiny microorganisms that clean up the environment by utilizing Green House Gases (GHG) to produce huge amounts of oil.
Microalgae are sunlight-driven cell factories that convert carbon dioxide to potential biofuels, foods, feeds and high-value bioactive compounds (Pharmaceuticals and Neutraceuticals). In addition, these photosynthetic microorganisms are useful in bioremediation applications and as nitrogen fixing biofertilizers. The idea of using microalgae as a source of fuel is not new but it is now being taken seriously because of the escalating price of petroleum and, more significantly, the emerging concern about global warming that is associated with burning fossil fuels. Unlike other oil crops, microalgae grow rapidly and many are exceedingly rich in oil. Microalgae commonly double their biomass within 24 h. Biomass doubling times during exponential growth are commonly as short as 3.5 h. Oil content in microalgae can exceed 80% by weight of dry biomass .Oil levels of 20–50% are quite common. Oil productivity, that is the mass of oil produced per unit volume of the micro algal broth per day, depends on the algal growth rate and the oil content of the biomass. Microalgae with high oil productivities are desired for producing biodiesel. ACHIEVEMENTS OF EBT ON MICROALGAL BASED BIOFUELS  EBT currently possess 64 different micro algal strains that produce ample amounts of lipids by cleaning up the flue gases
 EBT Algal strains have reported up to 54% of crude oil production on dry biomass basis
 EBT BRDC strains have the potential of yielding 245MT/ha/annum of dry biomass, and an oil yield of 132MT/ha/annum (IN LAB SCALE).
 Micro algae has a high oil content (minimum 37 %) and the oil has a high EPA/DHA content (greater than 40%) encapsulated within the micro-algal cells
 Standardization of protocols for extraction of micro algal crude oil would make the extraction possible up to 90%
 We have isolated a rapidly growing micro algal strain having 4 doublings per day and an oil yield of 50-60% under ambient culture conditions
 Standardized the protocol for separation of wet biomass and dried and recorded 21.3% of wet biomass to dry biomass
 Studies of growth curve for the EBT strains to minimize the doubling time of Algae
 The heavy metals present in the underutilized waste water are being effectively utilized by the microalgae for their growth and lipid production.
 Various growth parameters such as temperature, light intensity, photoperiod, pH, aeration and nutrient concentrations are being optimized for microalgal mass production
 The water considered as non-potable would serve as a good medium for microalgal multiplication
 EBT possess microalgal cultures that withstand high range of salinities in water  The mass cultivation of microalgae are done in cost effective outdoor ponds and polybags thereby utilizing ample amount of natural sunlight and greenhouse gases
 The Algal Neutraceuticals (Astaxanthin) obtained as a byproduct is of high value
 The Omega 3 oils obtained as a fraction of microalgal oil is of high pharmaceutical value thereby could be utilized as anti-cancerous, anti-inflammation, anti-HIV, anti-oxidant and treatment of cardio vascular problems
 Algae polysaccharides are also of pharmaceutical importance
 Green algae may just hold the answer to prevent future occurrences of hurricanes, tsunamis and other disasters stemming from climate changes PROCESSING:

Biodiesel processing technologies (1) Bio-diesel Existing transesterification process is under modification to suit the technology having higher FFA and phospholipids. But EBT goal is to use technologies other than trans-esterification process. Technologies in EBT pipeline is in joint venture with U.S.A
a. Nanocatalyst
b. Micro and Membrane reactors
c. Enzymatic transterification
d. Homogeneous catalyst (2) Glycerol Etherification Technology Glycerol etherification technology deals with the conversion and transformation of glycerol as a value added fuel product. This product act as an oxygenate additive to gasoline and Bio-fuels
It has a great potential in replacing chemically derived toxic oxygenates such as MTBE. The above technology has been jointly standardized and demonstrated along with our joint venture partners in U.S.A. (3) Renewable Aviation Turbine Fuels (ATF)
EBTI is in the process of developing bio-based aviation fuels. Bio-diesel can be used as aviation fuel to partially replace white kerosene, the fuel standard of bio-diesel can be enhanced to meet the ATF standards using suitable fuel additives. Bio-diesel can also be blended with ATF. (4) Bio-lubricants EBTI has also started R & D on bio-lubricants and we found the following merits using bio-lubricants from SVO
a. increase in engine life
b. 30% quantity reductions
c. more benefits to consumers Other applications of bio-lubricants a. Transformer oil
b. Insulating oil
c. Metallurgical oil
d. 2T oil
e. Hydraulic oil
f. Fluid oil Merits of EBTI’s Improved Technology
- No pollution
- No separation of FFA
- Process is in liquid form
- 0.7% less in cost of production Clean Development Mechanism (CDM)
CARBON CREDITS:
The following practices and activities have been evaluated for the purpose of exploring the potential for landowners to sequester carbon on and/or reduce greenhouse gas emissions related to their operation: High potential for carbon sequestration:
• Afforestation (new forest) on poorly stocked forest lands,
• Nutrient management,
• Biomass (cropland residues) energy source
• Afforestation on marginal cropland,
• Ethanol production and use,
• Residue management (no-till, direct seed),
• Biogas recovery, digesters,
• Afforestation on non-stocked forest land, Moderate potential for carbon sequestration:
• Short rotation woody crops,
• Prescribed grazing on rangeland,
• Cropland residue burning alternatives or techniques,
• Land conversion to permanent grass cover (similar to CRP),
• Rangeland planting,
• Windbreaks & shelterbelts,
• Afforestation on marginal pastureland,
• Conservation/restoration on private land, Low potential for carbon sequestration:
• Cover crops,
• Pastureland planting,
• Prescribed grazing on pasture land,
• Afforestation on pivot corners,
• Riparian forest buffers on non-forested land
• Riparian conservation/restoration on state land,
• Biodiesel production and use,
• Grassed waterways,
• Wetland construction and enhancement
 Clean Development Mechanism is the provision of the Kyoto protocol that governs project level carbon credit transactions between developed and developing countries  Eco-securities is one of the world's leading companies in the business of Originating, implementing and commercializing carbon credit  Eco-securities structures and guides greenhouse gas emission reduction projects through the Kyoto Protocol, acting as a principal between the projects and the buyers of carbon credits  Eco-securities works with Enhanced Bio-fuels and Technologies(I) Pvt Ltd., to create carbon credits from projects that reduce emissions of green house gases  Algae based bio-diesel production reduces green house gas emissions by consuming 2 tonnes of carbon dioxide for every tonne of bio-mass produced thereby releasing 1.6 tonnes of oxygen into the atmosphere  Carbon Credits acquired on Jatropha based bio-diesel production is IN US Dollars
Items Yield/Ha
in MT Carbon Credit
Unit (MT) Carbon Credit (MT) Valve
Total Value
BIODIESEL 3.6 2.2 7.92 25 198
CAKE
1)Methane gas
2)Burnt seed cake 6.4 1.4 8.96 25 224
Biomass 6 Mwh
Plantation 2000 trees@8kg per tree 16.0 25 400
Algae (wet biomass) 489 2.2 1076 25 26900
Algal Biodiesel 116 0.4 46.4 25 1160
Algal bioethanol 93 0.13 12.09 25 302.25
Total 1167.37 29,184.25 ENVIRONMENTAL BENEFITS IN COMPARISON TO PETROLEUM BASED FUELS INCLUDE: - -Biodiesel reduces emissions of carbon monoxide (CO) by approximately 50% and carbon dioxide by 78% on a net lifecycle basis because the carbon in biodiesel emissions is recycled from carbon that was in the atmosphere, rather than the carbon introduced from petroleum that was sequenced in the earth’s crust. However, it does produce more NOx emissions than standard diesel fuel. (Sheehan, 1998) - Biodiesel can reduce by as much as 20% the direct (tailpipe) emission of particulates, small particles of solid combustion products, on vehicles with particulate filters, compared with low-sulfur (<50 ppm) diesel, Particulate - Emissions as the result of production are reduced by around 50%, compared with fossil-sourced diesel. (Beer et al, 2004.) - Biodiesel has a higher cetane rating than petro-diesel, which can improve performance and clean up emissions compared to crude petro-diesel (with cetane lower than 40). - Biodiesel is biodegradable and non-toxic – the U.S Department of Energy confirms that biodiesel is less toxic than table salt and biodegradable as quickly as sugar. - In the United States, bio diesel is the only alternative fuel to have successfully completed the Health effect testing requirements (Tier I and Tier II) of the clean Air Act (1990). - The flash point of Bio diesel (>150° C) is significantly higher than that of petroleum diesel (64°C) or gasoline (- 45° C). The gel point of biodiesel varies depending on the proportion of different types of esters contained. However most biodiesel, including that made from soya bean oil, has some what higher gel and cloud point than petroleum diesel. In practice this often requires the heating of storage tanks, especially in cooler climates. - Pure biodiesel (B100) can be used in any petroleum diesel engine, though it is more commonly used in lower concentrations. Some areas have mandated ultra-low sulfur petro-diesel, which reduces the natural viscosity and lubricity of the fuel due to the removal of sulfur and certain other materials. Additives are required to make ULSD properly flow in engines, making biodiesel one popular alternatives. Ranges as low as 2% (B2) have -
been shown to restore lubricity. Many municipalities have started using 5% biodiesel (B5) in snow-removal equipment and other systems. SOCIAL BENEFITS OF BIODIESEL • Cleaner Burning &non toxin • Domestically Produced (Energy security) • Little to no infrastructure change needed to implement biodiesel. • Support regional farmers. • Creates new production jobs and a new industry • Insurance against oil embargos • Reduces dependency on foreign oil • Health is currently adversely affected from vehicle emissions. Diesel particulates are well known to be harmful. Anything that can reduce this can only be of social benefit. • Communities can become self sufficient with the local production and use of biodiesel strengthening independence and self sustainability. • The spectre of peak oil reminds us of the dependence our society has with fossil oil, so any alternatives are welcome. • No warns have been fought over Medicinal value:
The latex of Jatropha curcas contains an alkaloid known as jatrophine, which is proven to have anti-cancerous properties.
It is also used as an external application for skin diseases and rheumatism and for sores on domestic livestock.
The tender twigs of the plant are used for cleaning teeth, while the juice of the leaf is used as an external application for piles. The roots are reported to be used as an antidote for snake-bites
Insecticide/ pesticide:
The seeds are considered anthelimintic in Brazil, and the leaves are used for fumigating houses against bed-bugs. Also, the ether extract shows antibiotic activity against Stayphylococcus aureus and, Escherichia coli.
EBT recognizes the socio-economic value the cultivation of Jatropha curcas has for the highly decentralized rural communities, bridging the rich-poor divide, poverty reduction programmes, and as a pre-requisite for national fuel security and clean world. EBT aims to deliver end to end solutions for bio- energy designed to support all segments of the supply chain associated with the production of bio- diesel, bio- ethanol, bio- energy, bio- lubricants, bio- pesticides, bio- fertilizers and other bio-mass resources.
SCIENCE, ENGINEERING, TECHNICAL-IP
EBTI is able to supply a comprehensive suite of technology applications engineered to compliment the production of multiple feedstocks. Scalable, commercially viable technologies designed to support the production of bio fuels and extract a range of high value products from biomass. PHILOSOPHY
EBTI's philosophy has been to take cognisance of the advanced socio-economic policies promoted by the United Nations, G8 and world economic forums dedicated to the promotion of pro poverty reduction, anthropogenic green house gas capture, environmental conservation, and the globalisation of "fair" world trade; from a commercially viable stand point. As such, EBTI has emerged as a research and development organisation possessed of collaborative abilities for facilitating highly integrated complimentary commercial responses that add value and synergy to numerous scientific endeavours, national/international government and corporate development policy as well as financial markets. EBTI is constantly reviewing opportunities to facilitate the promotion of first class
investment into agriculture, technology and research for the production of carbon reduced or neutral energy. MANAGEMENT The EBTI executive has assembled a world class Board of Scientific Advisors, along with a group of established technology and process engineering companies that have entered into strategic partnerships for the design, development, operation and underwriting of a range of technologies identified to support the production of biofuels and processing of high value bi-products extracted from the biomass. EBTI's directors include senior executives drawn from the scientific, commercial, financial sectors of the Oil and Gas industry, international development policy organisations, government steering committees and world banking organisations.
DIRECTORS S. No. NAME DESIGNATION
1. Ganapathy Arumugam CEO & Group Science Director, Founder
2. Ramalakshmi Arumugam Director, Founder
NON-EXECUTIVE DIRECTORS
S. No. NAME DESIGNATION
1. M. Jayapragasam Director Research and Distinguished Scientist (Biotechnology and Biochemistry)
Former Professor, Tamil Nadu Agricultural University
2. Jag Sankar Professor North Carolina State A & T University and White House millennium
Researcher, Greensboro, USA
3. Joel L. Cuello Distinguished Scientist (Phycology) Associate Professor of The University of Arizona, USA.
4. G. Sakkari Commercial Operation - Projects and Govt coordination (EBTI)
5. G. Sankarasubramanian Chief Engineer Public Relations & Commercial Operations – Projects (EBTI)
6. P. Rajakumaran Chief Administrative officer & Project Engineer (EBTI)
7. N. Shanmuganathan - Finance and Project Management
SENIOR SCIENCE & MANAGEMENT TEAM
S. No. NAME DESIGNATION
1. S. R. Sree Rengasamy Principal Scientist, Genetic Engineering, Gene mapping & Molecular Biology, Distinguished Scientist, Formerly Director, TNAU, Coimbatore.
2. M. Muthusamy Principal Scientist, Engine Performances on Biofuels, Distinguished Scientist, Former Registrar, Bharathiar University, Director Factory Advise Govt of India.
3. C. Surendran Principal Scientist, Genetics and Plant Breeding, Distinguished Scientist, Former Dean, Tamil Nadu Agricultural University. 4. C. Dharmalingam Principal Scientist, Seed Science and Seed Technology, Distinguished Scientist, Former Dean, Tamil Nadu Agricultural University.
5. A. Gopalan Principal Scientist, Plant Breeding and Genetics, Distinguished Professor, Former Professor, Tamil Nadu Agricultural University, Coimbatore.
6. V. Murugappan Director – Commercial,
Former Director, Tamil Nadu Agricultural University.
7. A. Regupathy Principal Scientist, Plant Protection – Entomology Distinguished Scientist, Former Dean, Tamil Nadu Agricultural University.
8. R. Jayarajan Project Co-ordinator, Former Dean, Taminadu Agricultural University.
9. A. K. Gupta Principal Scientist, Processing and Chemical Engineer, Distinguished Scientist, Indian Institute of Petroleum, Dehuradun, New Delhi.
10. Guerry L. Grune P.A, Principal of CPS Biofuels, USA (Glycerin Etherification Technology)
11. R.S. Meenakshisundaram Chief Finance Officer
12. P. Kumaravadivelu Chief Operating Officer – Commercial Operations - Projects
13. P. Balasubramanian Chief Technical Officer – Commercial Operations - Plantation Management.
14. R. Gopalsamy Chief Technical Officer - Commercial
Operations - Projects and Nursery
management
15. R. Adaikalam Chief Technical Officer - Commercial
Operations – Projects
16. D. Ananda pradeep Senior Scientist - Phycology SCIENCE ADVISORY BOARD
S. No. NAME DESIGNATION
1. W. Selwamoorthy & Team Distinguished Scientist, Defence Research and Development Organisation, Govt of India, Chairman EBTI /DRDO /Army Biofuels
Programme
2. Ponnaiah Rethinam Former Executive Director, Asia Pacific
Coconut Community (APCC), Jakarta
3. T. N. Balasubramanian Distinguished Scientist (Irrigation & Microclimate Management) Former Professor Tamilnadu Agricultural University
4. S.V. Rao Distinguished Scientist (Crop Management & Genetics), Director, Jabalpur Agricultural
University
5. Santhos Gutru Distinguished Scientist (Biotechnology)
6. Ilangovan Kuppuswamy Distinguished Scientist (Nano Technology)
Mexico University
7. Nandhi. S. Bolan Distinguished Scientist (Phytoremediation) Professor Massey University, New Zealand
8. L. Kothandapani Distinguished Professor (Process Engineering) Former Professor Tamilnadu Agricultural University
9. Udayakumar Distinguished Scientist (Bioremediation)
Professor Gandhigarm Rural University.
10. K. Balraman Distinguished Scientist (Plant Pathology) Former Scientist Indian Institute of Horticulture Research
11. R.K. Sivanappans Distinguished Scientist (Irrigation and Water Management), TNAU PARTNERSHIPS S. No. UNIVERSITIES/INSTITUTIONS SPECIALIZATION
1. North Carolina State Agriculture Technology University, USA Nanotechnology & Genetic Engineering
2. Wagneningen University, Netherlands Gene mapping, Finger printing, Genetic Engineering & Molecular Biology
3. University of Arizona State, USA Genetic Engineering, Molecular Biology of Jatropha and Micro algae
4. Ministry of Defence, Govt of India, EBTI /DRDO /Army Biofuels programme
Defence Research and Development Organization, New Delhi
5. Jawaharlal Nehru University, New Delhi Micro Algae
6. University of Madras, Chennai Micro Algae VISION
In summary, EBTI's business is the delivery of end to end solutions for bio energy, designed to support all segments of the supply chain associated with the production of biodiesel, bio ethanol, bio energy, bio lubricants, bio pesticides, bio fertilizers and other high value commodities derived from the processing of various biomass resources. EBTI will extend its current research and development operations to introduce biotechnological feedstock supplies suitable for the production of biofuels, embracing a platform of complimentary technologies associated with the processing and production of high value bi-products. Application of tissue culture and methodologies for implementing multi-tier intercropping programs to existing coconut and palm tree plantations are ongoing projects which we expect to deliver significant value for the company over the next 2 years. EBTI has invested considerable time and money into researching the cultivation of Rapeseed from Russia, Belarus and the Ukraine as a short term solution for supplying vegetable oil for the production of biodiesel and RED. Recent feasibility studies proved that up to a 30% cost savings could be achieved from the engagement of contract farming arrangements
COMPANY BACKGROUND Green World Biotech, a Parent Company of Enhanced Biofuels and Technologies India Pvt Ltd., (EBTI) initiated various research and commercial projects on Agricultural and Horticultural crops since 1992. As a part of our research activities, Jatropha commonly known as Physic Nut , was planted as a supporting plant for Vanilla, in 1994. Since there was no demand for Biofuels earlier, the oil produced from Jatropha seeds were used for production of Soap. Later in 2004 we realized the importance of Biofuels and separated Jatropha from Green World Biotech and commenced a Biofuels Research and Development Centre, (BRDC) a Facility based on Non edible Feed Stocks such as Jatropha and Micro algae to create varieties, Standardization of Agronomical practices etc., to support the commercialization of Biofuel business. After having achieved successful, scalable results from the demonstration of Research & Development facility, BRDC was ready to turn the promise of research into commercial reality under the name of Enhanced Biofuels and Technologies India Pvt Ltd., (EBTI) registered on November 2006. As a primary recognition, our research activities were endorsed by our Former President of India Dr. A. P. J. Abdul Kalam on 5th April, 2006 on a meeting held at Rashtrapathi Bhavan, New Delhi. Based on the dedicated Research and Achievements thereafter, Memorandum of Understanding was signed between EBTI and Defence Research and Development Organization (DRDO) for the Development of Biofuel Technologies and Fuel types using Jatropha curcas and Algae Biomass on 15th September, 2006 at DRDO BHAWAN, New Delhi.
Material Acquisition Agreement was signed between EBTI and Plant Research International (PRI), Wageningen University, The Netherlands on 29th March, 2007. Memorandum of Understanding was signed between EBTI and The State Trading Corporation of India Limited (STC) on 29th May, 2007 to form a strategic partnership for the purpose of commercial exploitation of the IP held by EBTI, to develop a long term sustainable source of feedstock for the production of Biofuels and other associated byproducts. Collaboration Agreement was signed between EBTI and Platinum Energy Sdn Bhd, Malaysia on 30th May, 2007 in relation to the Development of Jatropha Plantations in Indonesia and Malaysia. Techno Commercial Supply Contract was signed between EBTI and Ashok Leyland Project Services Limited (ALPS), Chennai on May 2007 and both parties wished to enter into a sole and exclusive supply contract. Memorandum of Understanding was signed between EBTI and Platinum Energy (PE), USA on 22nd June, 2007 for extending technical and financial support for the research activities at EBTI. Techno Commercial Supply Contract was signed between EBTI and GEO CAPITAL on September 2007 and both parties wished to enter into a sole and exclusive supply contract. Memorandum of Understanding was signed between EBTI and The University of Arizona, on 30th October, 2007 for both the parties to work together towards mutually acceptable arrangements for selected and qualified employees of EBTI to enroll in graduate programs relating to Biofuels production in the Department of Agricultural and Biosystems Engineering at the UoFA. Memorandum of Understanding was signed between EBTI and Mascons, Srilanka on 24th October 2007 for commercialization of EBTI Biofuel Technology Memorandum of Understanding was signed between EBTI and Energy Allied International Corporation, Texas on 31st October 2007, for providing the information regarding the Plant Breeding Genetics for the implementation of a Jatropha agricultural farming program and the Breeding of Micro algae for Biofuels technologies and Biofuels Processing Technologies.
Memorandum of Understanding was signed between EBTI, and Van Der Horst Biodiesel (Singapore) Pte, Ltd, (VDHB) on 23rd November 2007 towards a technical contract where EBTI will provide the superior Jatropha curcas clones which yield up to 5 tons of crude oil per hectare upon maturity in the 4th and 5th year. EBTI will also perform feasibility studies, start-up nursery; develop detail, implementation plan for the identified plantation land by VDHB For further clarification, Please contact, Enhanced Biofuels and Technologies India P Ltd,
Biofuels Research and Development Centre,
5/10 C Alankar Garden,
GN Mills post,
Coimbatore 641 029
India.
Phone +91 422 2645640,2645660,2645630
Mobile + 91 94432 67360, 9443367360
Email: drga@ebtiplc.com, lakshmiga76@yahoo.co.uk
Web: www.ebtiplc.com

Research papers in Jatropha curcas & others crops
Dr. Yogendra K. Tripathi
Head, Research and Development
Leonid Bioenegry P Ltd
Sattur (a)International Journal: Yogendra K. Tripathi, Priyatansh Gurha, D.Ghosh, R.V.Kumar, H.K.Goswami, and Ved Prakash Yadav (2007). Phylogenetic cataloging of Isoetes species using random primers. Turkey Journal of Botany, 31,367-372. D.Ghosh, Yogendra K. Tripathi, Mujamdar, M.K., Ved Prakash Yadav (2006). Evaluation of salt tolerance capability of some cotton germplasm (Gossipium hirsutum l.). In press Tropical Agriculture, West Indies. R.V Kumar, Yogendra K. Tripathi, V. P. Yadav S.P. Ahlawat and V.K. Gupta (2006). Polyacrylamide Gel electrophoresis for Separation of Protein Isoforms in Jatropha curcas L. Communicated at Journal of Central European Agricultural Science. Ved Prakash Yadav, B.R. Ranwah, A.K. Nagda, S. N. Nigam,Yogendra K.Tripathi, R.V.Kumar S.P.Ahlawat and V.K.Gupta (2006). Rapid screening of Drought resistance in Peanut Germplasm of India By SPAD Chlorophyll Meter. Accepted Tropical Agriculture, West Indies. R.V. Kumar, S. H. Dar, S.P. Ahlawat, Ravinder Singh V.P. Yadav and Y.K. Tripathi (2006). Germination and growth pattern of seedlings in different accessions of Jatropha curcas L. Accepted. Tropical Agriculture, West Indies.
(b) National Journal: R.V. Kumar, Yogendra K. Tripathi, Ido Izhaki ,V. P. Yadav, and S.P. Ahlawat (2007). Intraspecific variation and interrelationships between morphology, nutritional content and enzymatic activity of Jatropha curcas L. Current Science, 95 (2): 239-243. Yogendra K. Tripathi, Priyatansh Gurha, D. Ghosh R.V. Kumar, H.K.Goswami, Rajeev Goswami, Ved Prakash Yadav and Neelu Singh (2005). Amplification Based Nucleic Acid Scanning of Isoetes pantii Using Random Primer. Plant Archives 5(2): 465-468. Yogendra K.Tripathi and D. Ghosh (2002). Isolation and RAPD PCR amplification of genomic DNA from white button mushroom Agaricus bisporus. Bionature 22(1): 99-103 Yogendra K.Tripathi and D. Ghosh (2003). Evaluation and development of high yielding hybrids by mycelial anastomosis in Agaricus bitorquis. Bionature 23(1):13-18 R.V Kumar, Yogendra K. Tripathi, V. P. Yadav and S.P. Ahlawat (2007). Study on laccase, peroxidase, cellulose and polyphenol oxidase enzyme activities in Jatropha curcas L. Indian J. Agroforestry 9(1) : 42-46.
R.V. Kumar, Yogendra K. Tripathi, V. P. Yadav and S.P. Ahlawat (2007). Molecular markers in Jatropha curcas L. improvement. TFRI, Jabalpur. R.V. Kumar, V. P. Yadav and Yogendra K. Tripathi, (2007). The role of wild species in improvement of cultivated groundnut (Archais hypogaea). Asian Journal of Horticulture Science 2 (1): 204-210. R.V. Kumar, Yogendra K Tripathi. Yadav, V. P., Ahlawat, S.P., Handa, A.K. and Ajit (2006). Population variation and interrelationships between Laccase enzyme activity and oil percentage of Jatropha curcas L. Indian J. Agroforestry 8(2): 47-53.
R.V. Kumar, Yogendra K.Tripathi , V. P.Yadav and S.P.Ahlawat, (2007). Oil percentage in Jatropha curcas L. germplasm of National Agroforestry Repository. Journal of Range Management and Agroforestry (Accepted). R.V. Kumar, Yogendra K. Tripathi, V. P. Yadav, S.P. Ahlawat and V.K. Gupta (2005). Estimation of peroxidase enzyme activity and genetic relationship in germplasm of Jatropha curcas L. Indian Journal of Biochemistry 18 (2): 75-81. R.V. Kumar, S.H. Dar, V. P. Yadav, Y. K. Tripathi and S.P. Ahlawat (2007) Genetic variability in Jatropha (Jatropha curcas L.) accessions. Indian Journal of Genetics and Plant Breeding (Communicated). R.V. Kumar, Y.K. Tripathi, V.P. Yadav Ravinder Singh, S.P. Ahlawat and SH. Dar (2005). Evaluation Of Genetic Divergence In Accessions Of Jatropha Curcas L. Accepted at TFRI, Jabalpur. R.V. Kumar, Y. K. Tripathi, V.P. Yadav, S.P. Ahlawat, and Neelu Singh (2006). Ratanjot (Jatropha curcas) mein laccase enzyme ki gatividhiyon ke aadhar per anuvanshik vividhta ka adhyayan. Bhartiya Krishi Anushandhan Patrika 21(2): 99-105.
M.C.Yadav, Y.K.Tripathi, R.N.Verma, R.C.Upadhyaya and B.L.Dhar (2003). Interstrainal hybridization for development of high yielding and false truffle resistant hybrid in Agaricus bitorquis Current Vistas in Mushroom Biology and Production, 10-16. R.V. Kumar, N.R. Choudhary, V.P. Yadav, and Yogendra K. Tripathi (2005). Phenotypic Variability and correlation of Jatropha gosipffolia L. Communicated.
Kumar, R.V., Dar, S. H , Yadav, V. P., Tripathi, Y. K., and Ahlawat, S. P. (2007). Genetic variability in Jatropha (Jatropha curcas L.) accessions. Journal of Range Management and Agroforestry (Accepted). R.V. Kumar, Yogendra K.Tripathi, V.P. Yadav, S.P. Ahlawat V.K.Gupta, A.K. Handa and Parul Shukla (2007). Study on intracellular cellulase and ligninase in germplasm of Jatropha curcas L. Journal of Range Management and Agroforestry 28(2): 258-259. R.V. Kumar, Y. K.Tripathi, V.P. Yadav, S.P. Ahlawat and V.K.Gupta (2007). Development of DNA isolation protocol for polymerase reaction based markers- assisted breeding in Jatropha curcas L. Agroforestry Newsletter 19(3) :1-2. Yogendra K. Tripathi, Priyatansh Gurha, H.K. Goswami and R.V. Kumar (2007). Biodiversity of Isoetes species in India and their Molecular characterization. International Conference on Biotechnological Approaches in Bioresource Management. Department of Biotechnology, Bharathidasan University, Triuchirappalli (T.N.), Jan 23-26,2007.
Yogendra K. Tripathi, Priyatansh Gurha, H.K. Goswami R.V. Kumar and Saurabh Kumar (2006). Specific enzymatic amplification of genomic DNA of Isoetes species using random primers. National Seminar on Biodiversity and its Conservation, Department of Botany, Kamla Nehru College, Korba (CG), November, 18-19,2006. Yogendra K. Tripathi and D.Ghosh.2002.Estimation of biodiversity of mushrooms by molecular tools 2002.National seminar on biodiversity and sustainable use of bioresource, Department of Liminology, B.U., Bhopal 10-12 October. R.V. Kumar, Yogendra K. Tripathi, V.P. Yadav, and S.P.Ahlawat (2006). Intraspecific variability in germplasm of Jatropha curcas L. National Symposium on livelihood and Biofuel Production. NRCAF, Jhansi.
R.V. Kumar, Yogendra K. Tripathi, V.P. Yadav, and S.P.Ahlawat (2006). Performance of Jatropha accessions for growth and seed yield. Training workshop on institutional, environmental, economical, technological and legal issue related to production possibilities of Bio-diesel from Jatropha curcas(Ratanjot) and Pogamia pinnata(Karanja). Amity School of Natural Resources & Sustainable Development, May 2-4,2006. R.V. Kumar, Yogendra K. Tripathi, V.P. Yadav, S.P.Ahlawat and V.K.Gupta (2006). Laccase enzyme polymorphism and genetic relationship in germplasm of Jatropha curcas L. National conference on forest biodiversity Resources :Exploitation Conservation & Management. Madurai Kamraj University, Madurai (T.N.) March 21-23, 2006. R.V. Kumar, Yogendra K. Tripathi, V.P. Yadav, S.P.Ahlawat and V.K.Gupta (2006). Peroxidase enzyme polymorphism and genetic relationship in germplasm of Jatropha curcas L. National seminar on Recent advance in forest sciences. Guru Ghasidas University, Bilaspur (CG) January 30-31, 2006, 67-68. R.V. Kumar, Yogendra K. Tripathi, V.P. Yadav, S.P.Ahlawat and V.K.Gupta (2006). Evaluation of genetic divergence in accessions of Jatropha curcas L. National seminar on Recent advance in forest sciences. Guru Ghasidas University, Bilaspur (CG) January 30-31,2006,163. R.V. Kumar, V.P. Yadav, Yogendra K. Tripathi, S.P.Ahlawat and V.K.Gupta (2006). Genetic Variability in Jatropha curcas L. National seminar on Recent advance in forest sciences. Guru Ghasidas University, Bilaspur (CG) January 30-31,2006,76-77. R.V. Kumar, Yogendra K. Tripathi , V.P. Yadav, , S.P.Ahlawat and V.K.Gupta (2006). Study on laccase, peroxidase, Polyphenol oxidase and cellulase in Jatropha curcas L. National Symposium on Tree Improvement for Sustainable Forestry, JNKV, Jabalpur (Accepted). Pushpendra K.Verma, Ved Prakash Yadav, M. Udaya Kumar,R. V. Kumar, Yogendra K. Tripathi and D. Ghosh 2005. Identification and classification of Coffea canephora L. based on DNA fingerprinting. . National seminar on medicinal and aromatic plants IGAU, Raipur (CG) Feb.26-27, 55. Kumar, R.V.; Choudhary, N.R.; Yadav,V.P. and Yogendra K. Tripathi (2005). Phenotypic Variability and correlation for morphological parameters in Jatropha gosipffolia L. National seminar on medicinal and aromatic plants IGAU, Raipur (CG) Feb.26-27, 189. R.V. Kumar, V. P. Yadav, Yogendra K. Tripathi, S.P. Ahlawat and V.K. Gupta (2006). Genetic divergence of seeds of Jatropha curcas L. National Seminar on Biodiesel : Emerging Trends . SDAU, Sardarkrushinagar, Gujarat. M.C.Yadav, R.N.Verma, R.C.Upadhyaya B.L.Dhar Y.K.Tripathi and D.Ghosh (2003). Interstrainal hybridization for development of high yielding and false truffle resistant hybrid in Agaricus bitorquis. Indian Mushroom Conference, T.N.A.U., Coimbatore.

Souvenir :
Jatropha- Jaiv Indhan ka Shrotra. NRCAF, Jhansi.. R.V.Kumar, S.P. Ahlawat, V.K.Gupta, A.K.Handa, Ved Prakash Yadav, and Yogendra K.Tripathi.
(d) Popular Articles (in Hindi):
V.P Yadav., S.K.Chaturvadi, R.V. Kumar and Yogendra K. Tripathi (2006). Importance of zerotileage techniques for improvement of rice and maize. (May- July) Rashtriya Krishi,.Pag 26-28. V.P.Yadav, R.V. Kumar and Yogendra K. Tripathi (2005). Hybrid varieties of important vegetable crops and their characters. (April- may) Khati Sudha,.Pag 5-8. V.P.Yadav, Nagda, A.K. Yogendra K. Tripathi and R.V. Kumar (2005). Increase productivity through water use efficiency and drought tolerant variety of groundnut. Khati Sudha. In Press.
V.P.Yadav, R.V. Kumar Yogendra K. Tripathi and S.K.Chaturvadi (2005). Importance of zerotileage techniques for improvement of rice and maize. (October- November) Khati sudha,.Pag 31-35.

In English: - (a) International:

R.V.Kumar, A.K. Handa,S.P. Alhawat,V. K. Gupta, Yadav,V.P. and Yogendra K. Tripathi(2005). Jatropha curcas miracle plant for Bio-diesel. SAARC, SAIC News latter, BARC Complex, Daka, Bangladesh 15(3): 4-6. R.V. Kumar, V. P. Yadav, Y. K. Tripathi, S.P. Ahlawat, V.K. Gupta and A.K. Handa Pongamia : An Alternative Source of Energy (2007). SAARC, SAIC News latter, BARC Complex, Daka, Bangladesh.
(b) National: Yogendra K. Tripathi, R.V. Kumar and, V.P Yadav (2006). Application and future prospectus of tissue and cell culture in crop improvement and crop production, Asian science, 1(2): 29-30. Yogendra K. Tripathi;, R.V. Kumar and V.P Yadav (2006). Antisense RNA Technology. Agriculture Update, 2(1): 1-2. R.V. Kumar, Yogendra K. Tripathi, V.P. Yadav, and S.P.Ahlawat (2007). Jatropha curcas L.: Renewable Source of Enegry. Communicated at D.S.T, New Delhi R.V. Kumar, S.P. Ahlawat, V. K. Gupta, A.K. Handa, Yogendra K. Tripathi and V.P. Yadav (2005). Jatropha curcas: Source for Biodiesel. Shilalipi.2 (1): 70-73. V.P.Yadav, S.K. Chaturvedi, R.V. Kumar and Yogendra K. Tripathi (2005). Pompousness Bt. Cotton technology in Indian Farmers. Agriculture Update 1(2): 12-14. (e) Book Chapter-
Agri. Biotechnology: Communicated
Yogendra K. Tripathi, R. V. Kumar, and V.P. Yadav. (2007). DNA Sequencing – Cracking of Genome.
Yogendra K. Tripathi, R. V. Kumar, V.P. Yadav and N. Singh. (2007). Opportunities and limitations of transgenic in agriculture. Ethnoforestry: The Future of Indian Forestry: In Press
R.V. Kumar Yogendra K. Tripathi S.P. Ahlawat and V.P. Yadav (2006). Genetic diversity of Jatropha species and prospectus in Agroforestry system.


Research Achievement Ø Role of lytic enzymes in the development of Jatropha curcas plant. (Publication no. 10) Ø Establishment the intercorrelations between the enzymes, nutritional compound and oil contents in Jatropha curcas. (Publication no. 4, 13) Ø Collected 200 hundred germplasm of Jatropha curcas for different geographical area of India. (Publication no.4, 10,13) Ø Studied of genetic variation and oil content percentage in Jatropha curcas of National Research Center Repository for Agroforestry, Jhansi. (Publication no.13, 14,15,16,17) Ø Establishment role of different pruning level on seed yield of Jatropha curcas. (Publication no.53) Ø Establishment role of different nutrition viz. Sulphur, N.P.K. in growth and seed yield of Jatropha curcas. (Publication no.53) Ø Studied of different agriculture crops with Jatropha curcas at different spacing (2x2, 3x3, 4x3 and 4x4) in agroforestry system. (Publication no.53) Ø Ph.D thesis on ““Evaluation of Combining ability and Molecular Characterization of hybrids in Jatropha curcas L. Ø Developed 156 intra specific hybrids of Jatropha curcas at National Research Center for Agroforestry, Jhansi. Ø Developed standard protocols for Protein profiling in Jatropha species. (Publication no. 3) Ø Developed protocols for DNA extraction from Jatropha curcas. (Publication no.23). Ø Developed protocols for RAPD technique in Jatropha curcas. (Publication no.11)
Research Responsibility at National Research Center for Agroforestry, Jhansi Ø Morphological Evaluation of germplasm of Jatropha curcas. Ø Independent responsibility for Development of Molecular Genetics laboratory at National Research Center for Agroforestry, Jhansi for Characterization of Jatropha and Karanja germplasm. Ø Independent responsibility of Biochemical Characterization of germplasm of Jatropha curcas. Ø Independent responsibility of Molecular assays of germplasm of Jatropha curcas. Ø Independent responsibility of Hybridization programme in Jatropha curcas. Ø Development of effective management programmes for cultivation of Jatropha curcas crop at Farmers and experimental field of National Research Center for Agroforestry, Jhansi Current Research Responsibility:
Ø Morphological Evaluation of germplasm of Jatropha curcas.
Ø Development of effective management programmes for cultivation of Jatropha curcas crop at experimental field of Leonid Bioenegry Ltd, Sattur.
Ø Independent responsibility of hybridization programme in Jatropha curcas.
Ø Independent responsibility for Development of laboratory.